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CXorf49B Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

CXorf49B Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all CXorf49B antibodies →
$504.00each
Catalog Number: LS-G126548-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human CXorf49B (NM_001145139), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Origin Species
Human
NCBI GenBank
NM_001145139

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
54.2 kda
Protein Type
Lysate
Amino Acid Sequence
1MSSPDKVSVC GAGFDLEGGK KAGSRTASPG APGAHSHGLD LGVPGSGDGK SESGFTDPEG
61FSFESESELI EQGRVVLWGR EGRPGTPVDD QGDVVDYSFY LADEPAAIVP PPSVQGHPFP
121EGAAAEGSAE NWADAEVGPS GRDVLGHSPG KWQQASAGRL HLCGPGPVRA WKNPERGSKS
181RWSLRVDPQQ PSAKGPTRLP THDSDSADES SDLPLMKVGI CRNEGSQAKP GSPKKRADTS
241RQASFHCKES YLPVPGRFLT SAPRGLTPVA ERPAVGELED SPQKKMQSRA WGKVEVRPSC
301SGAAAAGALP QGLSRRKMAG GKKSLGGASQ LALGRGFPAC GERLSAAPPE PATFPPFSGV
361RPQGMSKKPQ KPKHSSPGKK PAGRKTRESQ AAAREDNDPN RDEVPRAQLP THRPGLPRLS
421VRRGEFSSSD PNIRAPQLPG TSEPSAYSPG GLVPRRHAPS GNQQPPVHPP RPERQQQPPG
481AQGCPRCIWL QREIEDLTQQ LAAMQFLTDK FQDL
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

514 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.