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CXorf23 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

CXorf23 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all CXorf23 antibodies →
$725.00each
Catalog Number: LS-G103771-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human CXorf23 (NM_198279), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Origin Species
Human

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
80.2 kDa
Protein Type
Lysate
Amino Acid Sequence
1MARSRSRSPR WKHRSLSPVP RNAEHYKQRH SHGHYGCEYR KDPKRPVAWR MDSEKHGQSK
61PRIPSRGNIY YQSYEHRSPS PNIRNSLENV YMYKPHRGYS PGRGDSNRRA QYMPKYSEGI
121PYKEHERNSY PQKVQGGHSP DDHRVRGSGK GGKPPQRSIA DSFRFEGKWH EDELRHQRIQ
181EEKYSQSTRR GSEDFETRSS FQKRYPEDRD FRKYGHTSKR PKDVERYESR EPARNPKWKP
241EHSLPPYQED TDQWNLGPQT YRHAEREHPE TSSATKVSYD YRHKRPKLLD GDQDFSDGRT
301QKYCKEEDRK YSFQKGPLNR ELDCFNTGRG RETQDGQVKE PFKPSKKDSI ACTYSNKNDV
361DLRSSNDKWK EKIKKEGDCR KESNSSSNQL DKSQKLPDVK PSPINLRKKS LTVKVDVKKT
421VDTFRVASSY STERQMSHDL VAVGRKSENF HPVFEHLDST QNTENKPTGE FAQEIITIIH
481QVKANYFPSP GITLHERFST MQDIHKADVN EIPLNSDPEI HRRIDMSLAE LQSKQAVIYE
541SEQTLIKIID PNDLRHDIER RRKERLQNED EHIFHIASAA ERDDQNSSFS KNYTTQRKDI
601ITHKPFEVEG NHRNTRVRPF KSNFRGGRCQ PNYKSGLVQK SLYIQAKYQR LRFTGPRGFI
661THKFRERLMR KKKEYTDVAT GI
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

682 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.