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CXCR1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

CXCR1 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G99099-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human CXCR1 (NM_000634), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
C-C | Chemokine c-x-c receptor 1 | CKR-1 | CXCR1 | CMKAR1 | CD128 | CD181 | CD181 antigen | CXCR-1 | CXC-R1 | IL-8R A | Il8 receptor a | Interleukin 8 receptor a | Interleukin 8 receptor alpha | Interleukin 8 receptor | alpha | Interleukin-8 receptor type A | IL8RBA | Interleukin-8 receptor type 1 | C-C-CKR-1 | CDw128a | IL-8 receptor type 1 | IL8R1 | IL8RA
NCBI GenBank
NM_000634
SwissProt
P25024

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
39.6 kda
Protein Type
Lysate
Amino Acid Sequence
1MSNITDPQMW DFDDLNFTGM PPADEDYSPC RLETETLNKY VVIIAYALVF LLSLLGNSLV
61MLVILYSRVG RSVTDVYLLN LALADLLFAL TLPIWAASKV NGWIFGTFLC KVVSLLKEVN
121FYSGILLLAC ISVDRYLAIV HATRTLTQKR HLVKFVCLGC WGLSMNLSLP FFLFRQAYHP
181NNSSPVCYEV LGNDTAKWRM VLRILPHTFG FIVPLFVMLF CYGFTLRTLF KAHMGQKHRA
241MRVIFAVVLI FLLCWLPYNL VLLADTLMRT QVIQESCERR NNIGRALDAT EILGFLHSCL
301NPIIYAFIGQ NFRHGFLKIL AMHGLVSKEF LARHCVTSYT SSSVNVSSNL
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

350 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.