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CWH43 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

CWH43 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G109601-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human CWH43 (NM_025087), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
CWH43 | PGAP2-interacting protein | PGAP2IP | CWH43-C | FLJ21511
Origin Species
Human
NCBI GenBank
NM_025087
SwissProt
Q9H720

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
78.4 kDa
Protein Type
Lysate
Amino Acid Sequence
1MPSLWREILL ESLLGCVSWS LYHDLGPMIY YFPLQTLELT GLEGFSIAFL SPIFLTITPF
61WKLVNKKWML TLLRIITIGS IASFQAPNAK LRLMVLALGV SSSLIVQAVT WWSGSHLQRY
121LRIWGFILGQ IVLVVLRIWY TSLNPIWSYQ MSNKVILTLS AIATLDRIGT DGDCSKPEEK
181KTGEVATGMA SRPNWLLAGA AFGSLVFLTH WVFGEVSLVS RWAVSGHPHP GPDPNPFGGA
241VLLCLASGLM LPSCLWFRGT GLIWWVTGTA SAAGLLYLHT WAAAVSGCVF AIFTASMWPQ
301TLGHLINSGT NPGKTMTIAM IFYLLEIFFC AWCTAFKFVP GGVYARERSD VLLGTMMLII
361GLNMLFGPKK NLDLLLQTKN SSKVLFRKSE KYMKLFLWLL VGVGLLGLGL RHKAYERKLG
421KVAPTKEVSA AIWPFRFGYD NEGWSSLERS AHLLNETGAD FITILESDAS KPYMGNNDLT
481MWLGEKLGFY TDFGPSTRYH TWGIMALSRY PIVKSEHHLL PSPEGEIAPA ITLTVNISGK
541LVDFVVTHFG NHEDDLDRKL QAIAVSKLLK SSSNQVIFLG YITSAPGSRD YLQLTEHGNV
601KDIDSTDHDR WCEYIMYRGL IRLGYARISH AELSDSEIQM AKFRIPDDPT NYRDNQKVVI
661DHREVSEKIH FNPRFGSYKE GHNYENNHHF HMNTPKYFL
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

699 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.