Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
CTSZ / Cathepsin Z Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G99415-100
Catalog Number: LS-G99415
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Cathepsin Z(CTSZ) (NM_001336), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Cathepsin IV | Cathepsin P | Cathepsin Z1 | Carboxypeptidase LB | CTSZ | Cathepsin X | Cathepsin Y | CTSX | Lysosomal carboxypeptidase B | Preprocathepsin P | Cathepsin B2 | Cathepsin Z | Cysteine-type carboxypeptidase
- NCBI GenBank
- NM_001336
- SwissProt
- Q9UBR2
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 33.87 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MARRGPGWRP LLLLVLLAGA AQGGLYFRRG QTCYRPLRGD GLAPLGRSTY PRPHEYLSPA61DLPKSWDWRN VDGVNYASIT RNQHIPQYCG SCWAHASTSA MADRINIKRK GAWPSTLLSV121QNVIDCGNAG SCEGGNDLSV WDYAHQHGIP DETCNNYQAK DQECDKFNQC GTCNEFKECH181AIRNYTLWRV GDYGSLSGRE KMMAEIYANG PISCGIMATE RLANYTGGIY AEYQDTTYIN241HVVSVAGWGI SDGTEYWIVR NSWGEPWGER GWLRIVTSTY KDGKGARYNL AIEEHCTFGD301PIVTRTRPLEQKLISEEDLAANDILDYKDDDDKV
303 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.