Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
CTSC / Cathepsin C / JP Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G99578-100
Catalog Number: LS-G99578
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human DPP1(CTSC) (NM_001814), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Cathepsin J | Dipeptidyl peptidase 1 | Dipeptidyl peptidase I | DPP-I | Dipeptidyl transferase | Dipeptidyl-peptidase I | CPPI | CTSC | DPPI | DPP1 | HMS | JP | PDON1 | PLS | JPD | Cathepsin C | PALS
- NCBI GenBank
- NM_001814
- SwissProt
- P53634
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 51.84 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MGAGPSLLLA ALLLLLSGDG AVRCDTPANC TYLDLLGTWV FQVGSSGSQR DVNCSVMGPQ61EKKVVVYLQK LDTAYDDLGN SGHFTIIYNQ GFEIVLNDYK WFAFFKYKEE GSKVTTYCNE121TMTGWVHDVL GRNWACFTGK KVGTASENVY VNTAHLKNSQ EKYSNRLYKY DHNFVKAINA181IQKSWTATTY MEYETLTLGD MIRRSGGHSR KIPRPKPAPL TAEIQQKILH LPTSWDWRNV241HGINFVSPVR NQASCGSCYS FASMGMLEAR IRILTNNSQT PILSPQEVVS CSQYAQGCEG301GFPYLIAGKY AQDFGLVEEA CFPYTGTDSP CKMKEDCFRY YSSEYHYVGG FYGGCNEALM361KLELVHHGPM AVAFEVYDDF LHYKKGIYHH TGLRDPFNPF ELTNHAVLLV GYGTDSASGM421DYWIVKNSWG TGWGENGYFR IRRGTDECAI ESIAVAATPI PKLTRTRPLEQKLISEEDLAANDILDYKDDDDKV
463 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.