Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
CTSA / Cathepsin A Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G129888-100
Catalog Number: LS-G129888
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human CTSA(CTSA) (NM_001167594), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Beta-galactosidase 2 | Carboxypeptidase-L | Carboxypeptidase C | CTSA | GSL | GLB2 | Lysosomal carboxypeptidase A | PPCA | Protective protein cathepsin A | Protective protein | Urinary kininase | PPGB | Carboxypeptidase L | Cathepsin A | Cathepsin A (PPCA) | Deamidase | Lysosomal protective protein | NGBE
- Origin Species
- Human
- NCBI GenBank
- NM_000308
- SwissProt
- P10619
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 54.7 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MTSSPRAPPG EQGRGGAEMI RAAPPPLFLL LLLLLLLVSW ASRGEAAPDQ DEIQRLPGLA61KQPSFRQYSG YLKGSGSKHL HYWFVESQKD PENSPVVLWL NGGPGCSSLD GLLTEHGPFL121IANVLYLESP AGVGFSYSDD KFYATNDTEV AQSNFEALQD FFRLFPEYKN NKLFLTGESY181AGIYIPTLAV LVMQDPSMNL QGLAVGNGLS SYEQNDNSLV YFAYYHGLLG NRLWSSLQTH241CCSQNKCNFY DNKDLECVTN LQEVARIVGN SGLNIYNLYA PCAGGVPSHF RYEKDTVVVQ301DLGNIFTRLP LKRMWHQALL RSGDKVRMDP PCTNTTAAST YLNNPYVRKA LNIPEQLPQW361DMCNFLVNLQ YRRLYRSMNS QYLKLLSSQK YQILLYNGDV DMACNFMGDE WFVDSLNQKM421EVQRRPWLVK YGDSGEQIAG FVKEFSHIAF LTIKGAGHMV PTDKPLAAFT MFSRFLNKQP481YTRTRPLEQKLISEEDLAANDILDYKDDDDKV
481 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.