Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
CTNND1 / p120 Catenin Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G119899-100
Catalog Number: LS-G119899
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human CTNND1 (NM_001085467), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- CAS | Catenin delta-1 | CTNND1 | CTNND | KIAA0384 | p120 | p120(CAS) | p120(CTN) | p120CAS | p120CTN | p120 catenin | p120-catenin
- Origin Species
- Human
- NCBI GenBank
- NM_001331
- SwissProt
- O60716
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 92.5 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MQEPGQIVET YTEEDPEGAM SVVSVETSDD GTTRRTETTV KKVVKTVTTR TVQPVAMGPD61GLPVDASSVS NNYIQTLGRD FRKNGNGGPG PYVGQAGTAT LPRNFHYPPD GYSRHYEDGY121PGGSDNYGSL SRVTRIEERY RPSMEGYRAP SRQDVYGPQP QVRVGGSSVD LHRFHPEPYG181LEDDQRSMGY DDLDYGMMSD YGTARRTGTP SDPRRRLRSY EDMIGEEVPS DQYYWAPLAQ241HERGSLASLD SLRKGGPPPP NWRQPELPEV IAMLGFRLGA VKSNAAAYLQ HLCYRNDKVK301TDVRKLKGIP VLVGLLDHPK KEVHLGACGA LKNISFGRDQ DNKIAIKNCD GVPALVRLLR361KARDMDLTEV ITGTLWNLSS HDSIKMEIVD HALHALTDEV IIPHSGWERE PNEDCKPRHI421EWESVLTNTA GCLRNVSSER SEARRKLREC DGLVDALIFI VQAEIGQKDS DSKLVENCVC481LLRNLSYQVH REIPQAERYQ EAAPNVANNT GPHAASCFGA KKGKGKKPIE DPANDTVDFP541KRTSPARGYE LLFQPEVVRI YISLLKESKT PAILEASAGA IQNLCAGRWT YGRYIRSALR601QEKALSAIAD LLTNEHERVV KAASGALRNL AVDARNKELI GKHAIPNLVK NLPGGQQNSS661WNFSEDTVIS ILNTINEVIA ENLEAAKKLR ETQGIEKLVL INKSGNRSEK EVRAAALVLQ721TIWGYKELRK PLEKEGWKKS DFQVNLNNAS RSQSSHSYDD STLPLIDRNQ KSDKKPDREE781IQMSNMGSNT KSLDNNYSTP NERGDHNRTL DRSGDLGDME PLKGTTPLMQ KITRTRPLEQKLISEEDLAANDILDYKDDDDKV
832 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.