Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
CTNND1 / p120 Catenin Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G119894-100
Catalog Number: LS-G119894
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human CTNND1 (NM_001085461), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- CAS | Catenin delta-1 | CTNND1 | CTNND | KIAA0384 | p120 | p120(CAS) | p120(CTN) | p120CAS | p120CTN | p120 catenin | p120-catenin
- Origin Species
- Human
- NCBI GenBank
- NM_001331
- SwissProt
- O60716
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 104 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MDDSEVESTA SILASVKEQE AQFEKLTRAL EEERRHVSAQ LERVRVSPQD ANPLMANGTL61TRRHQNGRFV GDADLERQKF SDLKLNGPQD HSHLLYSTIP RMQEPGQIVE TYTEEDPEGA121MSVVSVETSD DGTTRRTETT VKKVVKTVTT RTVQPVAMGP DGLPVDASSV SNNYIQTLGR181DFRKNGNGGP GPYVGQAGTA TLPRNFHYPP DGYSRHYEDG YPGGSDNYGS LSRVTRIEER241YRPSMEGYRA PSRQDVYGPQ PQVRVGGSSV DLHRFHPEPY GLEDDQRSMG YDDLDYGMMS301DYGTARRTGT PSDPRRRLRS YEDMIGEEVP SDQYYWAPLA QHERGSLASL DSLRKGGPPP361PNWRQPELPE VIAMLGFRLG AVKSNAAAYL QHLCYRNDKV KTDVRKLKGI PVLVGLLDHP421KKEVHLGACG ALKNISFGRD QDNKIAIKNC DGVPALVRLL RKARDMDLTE VITGTLWNLS481SHDSIKMEIV DHALHALTDE VIIPHSGWER EPNEDCKPRH IEWESVLTNT AGCLRNVSSE541RSEARRKLRE CDGLVDALIF IVQAEIGQKD SDSKLVENCV CLLRNLSYQV HREIPQAERY601QEAAPNVANN TGPHAASCFG AKKGKGKKPI EDPANDTVDF PKRTSPARGY ELLFQPEVVR661IYISLLKESK TPAILEASAG AIQNLCAGRW TYGRYIRSAL RQEKALSAIA DLLTNEHERV721VKAASGALRN LAVDARNKEL IGKHAIPNLV KNLPGGQQNS SWNFSEDTVI SILNTINEVI781AENLEAAKKL RETQGIEKLV LINKSGNRSE KEVRAAALVL QTIWGYKELR KPLEKEGWKK841SDFQVNLNNA SRSQSSHSYD DSTLPLIDRN QKSDKKPDRE EIQMSNMGSN TKSLDNNYST901PNERGDHNRT LDRSGDLGDM EPLKGTTPLM QKISGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV
933 aa + 34 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.