Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
CTNNA3 / Alpha-3 Catenin Protein (Over-Expression Lysate Myc-DDK (Flag))
ProteinLSBio Guarantee
| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G114366-100
Catalog Number: LS-G114366
For bulk orders or custom formulations:
Request a QuoteOverview
- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human CTNNA3 (NM_013266), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Alpha-T-catenin | Cadherin-associated protein | Catenin alpha-3 | CTNNA3 | Alpha T-catenin | Alpha-catenin-like protein | VR22
- Origin Species
- Human
- NCBI GenBank
- NM_013266
- SwissProt
- Q9UI47
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 99.6 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MSAETPITLN IDPQDLQVQT FTVEKLLEPL IIQVTTLVNC PQNPSSRKKG RSKRASVLLA61SVEEATWNLL DKGEKIAQEA TVLKDELTAS LEEVRKESEA LKVSAERFTD DPCFLPKREA121VVQAARALLA AVTRLLILAD MIDVMCLLQH VSAFQRTFES LKNVANKSDL QKTYQKLGKE181LENLDYLAFK RQQDLKSPNQ RDEIAGARAS LKENSPLLHS ICSACLEHSD VASLKASKDT241VCEEIQNALN VISNASQGIQ NMTTPPEPQA ATLGSALDEL ENLIVLNPLT VTEEEIRPSL301EKRLEAIISG AALLADSSCT RDLHRERIIA ECNAIRQALQ DLLSEYMNNA GKKERSNTLN361IALDNMCKKT RDLRRQLRKA IIDHVSDSFL DTTVPLLVLI EAAKNGREKE IKEYAAIFHE421HTSRLVEVAN LACSMSTNED GIKIVKIAAN HLETLCPQII NAALALAARP KSQAVKNTME481MYKRTWENHI HVLTEAVDDI TSIDDFLAVS ESHILEDVNK CIIALRDQDA DNLDRAAGAI541RGRAARVAHI VTGEMDSYEP GAYTEGVMRN VNFLTSTVIP EFVTQVNVAL EALSKSSLNV601LDDNQFVDIS KKIYDTIHDI RCSVMMIRTP EELEDVSDLE EEHEVRSHTS IQTEGKTDRA661KMTQLPEAEK EKIAEQVADF KKVKSKLDAE IEIWDDTSND IIVLAKNMCM IMMEMTDFTR721GKGPLKHTTD VIYAAKMISE SGSRMDVLAR QIANQCPDPS CKQDLLAYLE QIKFYSHQLK781ICSQVKAEIQ NLGGELIMSA LDSVTSLIQA AKNLMNAVVQ TVKMSYIAST KIIRIQSPAG841PRHPVVMWRM KAPAKKPLIK REKPEETCAA VRRGSAKKKI HPLQVMSEFR GRQIYTRTRPLEQKLISEEDLAANDILDYKDDDDKV
895 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.