Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
CTBP1 / CTBP Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G121412-100
Catalog Number: LS-G121412
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human CTBP1 (NM_001012614), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- BARS | C-terminal binding protein 1 | C-terminal-binding protein 1 | CTBP | CTBP1
- Origin Species
- Human
- NCBI GenBank
- NM_001328
- SwissProt
- Q13363
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 46.2 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MSGVRPPIMN GPLHPRPLVA LLDGRDCTVE MPILKDVATV AFCDAQSTQE IHEKVLNEAV61GALMYHTITL TREDLEKFKA LRIIVRIGSG FDNIDIKSAG DLGIAVCNVP AASVEETADS121TLCHILNLYR RATWLHQALR EGTRVQSVEQ IREVASGAAR IRGETLGIIG LGRVGQAVAL181RAKAFGFNVL FYDPYLSDGV ERALGLQRVS TLQDLLFHSD CVTLHCGLNE HNHHLINDFT241VKQMRQGAFL VNTARGGLVD EKALAQALKE GRIRGAALDV HESEPFSFSQ GPLKDAPNLI301CTPHAAWYSE QASIEMREEA AREIRRAITG RIPDSLKNCV NKDHLTAATH WASMDPAVVH361PELNGAAYRY PPGVVGVAPT GIPAAVEGIV PSAMSLSHGL PPVAHPPHAP SPGQTVKPEA421DRDHASDQLTRTRPLEQKLISEEDLAANDILDYKDDDDKV
429 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.