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CTAGE6 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

CTAGE6 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G104625-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human CTAGE6 (NM_178561), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Origin Species
Human

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
87.7 kDa
Protein Type
Lysate
Amino Acid Sequence
1MEEPGATPQP YLGLVLEELR RVVAALPESM RPDENPYGFP SELVVCAAVI GFFVVLLFLW
61RSFRSVRSRL YVGREQKLGA TLSGLIEEKC KLLEKFSLIQ KEYEGYEVES SLEDASFEKA
121AAEEARSLEA TCEKLNRSNS ELEDEILCLE KDLKEEKSKH SQQDELMADI SKSIQSLEDE
181SKSLKSQIAE AKIICKTFKM SEERRAIAIK DALNENSQLQ TSHKQLFQQE AEVWKGEVSE
241LNKQKITFED SKVHAEQVLN DKENHIKTLT GHLPMMKDQA AVLEEDTTDD DNLELEVNSQ
301WENGANLDDP LKGALKKLIH AAKLNVSLKS LEGERNHIII QLSEVDKTKE ELTEHIKNLQ
361TQQESLQSEN IYFESENQKL QQKLKIMTEF YQEDEMKLYR KLTVEENYRI EGEEKLSKVE
421EKLSRATEQL ETYRKLAKDL EEELERTVHF YQKQVISYEK RGHDNWLAAR TAERNLSDLR
481KENAHNKQKL TETELKFELL EKDPNALDVS NTAFGREHAP NGPAPLGQRS SETRAFLSPQ
541TLLEDPLGLS PVLPEGGGRG PRGPGNPLDH QITNERGEPS CDRLTDPHRA PSDTGSLSSP
601VEQDCKMMFP PPGQSYPDSA LPPQREDRFY SNSERLSGSA EPRSFKMTSL DKMDGSMPSE
661MESSRNDAKD DLGNLNVPDS SLPAENEATG PGFIPPPLAP VRGPLFPVDT RGPFMRRGPP
721FPPPPPGTMF GASRGYFPPR DFPGPPHAPF AMRNIYPPRG LPPYFHPRPG FYPNPAF
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

777 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.