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CTAGE15 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

CTAGE15 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all CTAGE15 antibodies →
$504.00each
Catalog Number: LS-G121875-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human CTAGE15 (NM_001008747), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Origin Species
Human
NCBI GenBank
NM_001008747

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
83 kDa
Protein Type
Lysate
Amino Acid Sequence
1MCSCYWIFCC SPFFVEKFRS VRSRLYVGRE QKLGATLSGL IEEKCKLLEK FSLIQKEYEG
61YEVESSLEDA SFEKAAAEEA RSLEATCEKL NRSNSELEDE ILCLEKDLKE EKSKHSQQDE
121LMADISKSIQ SLEDESKSLK SQIAEAKIIC KTFKMSEERR AIAIKDALNE NSQLQTSHKQ
181LFQQEAEVWK GEVSELNKQK ITFEDSKVHA EQVLNDKENH IKTLTGHLPM MKDQAAVLEE
241DTTDDDNLEL EVNSQWENGA NLDDPLKGAL KKLIHAAKLN VSLKSLEGER NHIIIQLSEV
301DKTKEELTEH IKNLQTQQES LQSENIYFES ENQKLQQKLK IMTEFYQEDE MKLYRKLTVE
361ENYRIEGEEK LSKVEEKLSR ATEQLETYRK LAKDLEEELE RTVHFYQKQV ISYEKRGHDN
421WLAARTAERN LSDLRKENAH NKQKLTETEL KFELLEKDPN ALDVSNTAFG REHAPNGPAP
481LGQRSSETRA FLSPQTLLED PLGLSPVLPE GGGRGPRGPG NPLDHQITNE RGEPSCDRLT
541DPHRAPSDTG SLSSPVEQDC KMMFPPPGQS YPDSALPPQR EDRFYSNSER LSGSAEPRSF
601KMTSLDKMDG SMPSEMESSR NDAKDDLGNL NVPDSSLPAE NEATGPGFIP PPLAPVRGPL
661FPVDTRGPFM RRGPPFPPPP PGTMFGASRG YFPPRDFPGP PHAPFAMRNI YPPRGLPPYF
721HPRPGFYPNP AF
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

732 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.