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CSTF2T Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

CSTF2T Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G113374-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human CSTF2T (NM_015235), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
CstF-64T | CSTF2T | CF-1 64 kDa subunit tau | CSTF 64 kDa subunit tau | KIAA0689 | TauCstF-64
Origin Species
Human
NCBI GenBank
NM_015235
SwissProt
Q9H0L4

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
64.3 kDa
Protein Type
Lysate
Amino Acid Sequence
1MSSLAVRDPA MDRSLRSVFV GNIPYEATEE QLKDIFSEVG SVVSFRLVYD RETGKPKGYG
61FCEYQDQETA LSAMRNLNGR EFSGRALRVD NAASEKNKEE LKSLGPAAPI IDSPYGDPID
121PEDAPESITR AVASLPPEQM FELMKQMKLC VQNSHQEARN MLLQNPQLAY ALLQAQVVMR
181IMDPEIALKI LHRKIHVTPL IPGKSQSVSV SGPGPGPGPG LCPGPNVLLN QQNPPAPQPQ
241HLARRPVKDI PPLMQTPIQG GIPAPGPIPA AVPGAGPGSL TPGGAMQPQL GMPGVGPVPL
301ERGQVQMSDP RAPIPRGPVT PGGLPPRGLL GDAPNDPRGG TLLSVTGEVE PRGYLGPPHQ
361GPPMHHASGH DTRGPSSHEM RGGPLGDPRL LIGEPRGPMI DQRGLPMDGR GGRDSRAMET
421RAMETEVLET RVMERRGMET CAMETRGMEA RGMDARGLEM RGPVPSSRGP MTGGIQGPGP
481INIGAGGPPQ GPRQVPGISG VGNPGAGMQG TGIQGTGMQG AGIQGGGMQG AGIQGVSIQG
541GGIQGGGIQG ASRQGGSQPS SFSPGQSQVT PQDQEKAALI MQVLQLTADQ IAMLPPEQRQ
601SILILKEQIQ KSTGAS
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

616 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.