Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
CSRNP2 / FAM130A1 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G109396-100
Catalog Number: LS-G109396
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human FAM130A1(CSRNP2) (NM_030809), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- C12orf2 | C12orf22 | CSRNP-2 | CSRNP2 | FAM130A1 | PPP1R72 | Protein FAM130A1 | TAIP-12 | TAIP12
- Origin Species
- Human
- NCBI GenBank
- NM_030809
- SwissProt
- Q9H175
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 59.4 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MDAFTGSGLK RKFDDVDVGS SVSNSDDEIS SSDSADSCDS LNPPTTASFT PTSILKRQKQ61LRRKNVRFDQ VTVYYFARRQ GFTSVPSQGG SSLGMAQRHN SVRSYTLCEF AQEQEVNHRE121ILREHLKEEK LHAKKMKLTK NGTVESVEAD GLTLDDVSDE DIDVENVEVD DYFFLQPLPT181KRRRALLRAS GVHRIDAEEK QELRAIRLSR EECGCDCRLY CDPEACACSQ AGIKCQVDRM241SFPCGCSRDG CGNMAGRIEF NPIRVRTHYL HTIMKLELES KRQVSRPAAP DEEPSPTASC301SLTGAQGSET QDFQEFIAEN ETAVMHLQSA EELERLKAEE DSSGSSASLD SSIESLGVCI361LEEPLAVPEE LCPGLTAPIL IQAQLPPGSS VLCFTENSDH PTASTVNSPS YLNSGPLVYY421QVEQRPVLGV KGEPGTEEGS ASFPKEKDLN VFSLPVTSLV ACSSTDPAAL CKSEVGKTPT481LEALLPEDCN PEEPENEDFH PSWSPSSLPF RTDNEEGCGM VKTSQQNEDR PPEDSSLELP541LAVTRTRPLEQKLISEEDLAANDILDYKDDDDKV
543 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.