Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
CSRNP1 / AXUD1 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G108483-100
Catalog Number: LS-G108483
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human AXUD1(CSRNP1) (NM_033027), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- AXUD1 | CSRNP-1 | CSRNP1 | FAM130B | Protein URAX1 | TAIP3 | AXIN1 up-regulated 1 | TAIP-3 | URAX1
- Origin Species
- Human
- NCBI GenBank
- NM_033027
- SwissProt
- Q96S65
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 63.3 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MTGLLKRKFD QLDEDNSSVS SSSSSSGCQS RSCSPSSSVS RAWDSEEEGP WDQMPLPDRD61FCGPRSFTPL SILKRARRER PGRVAFDGIT VFYFPRCQGF TSVPSRGGCT LGMALRHSAC121RRFSLAEFAQ EQARARHEKL RQRLKEEKLE MLQWKLSAAG VPQAEAGLPP VVDAIDDASV181EEDLAVAVAG GRLEEVSFLQ PYPARRRRAL LRASGVRRID REEKRELQAL RQSREDCGCH241CDRICDPETC SCSLAGIKCQ MDHTAFPCGC CREGCENPMG RVEFNQARVQ THFIHTLTRL301QLEQEAESFR ELEAPAQGSP PSPGEEALVP TFPLAKPPMN NELGDNSCSS DMTDSSTASS361SASGTSEAPD CPTHPGLPGP GFQPGVDDDS LARILSFSDS DFGGEEEEEE EGSVGNLDNL421SCFHPADIFG TSDPGGLASW THSYSGCSFT SGILDENANL DASCFLNGGL EGSREGSLPG481TSVPPSMDAG RSSSVDLSLS SCDSFELLQA LPDYSLGPHY TSQKVSDSLD NIEAPHFPLP541GLSPPGDASS CFLESLMGFS EPAAEALDPF IDSQFEDTVP ASLMEPVPVTRTRPLEQKLISEEDLAANDILDYKDDDDKV
589 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.