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CSPP1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

CSPP1 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all CSPP1 antibodies →
$725.00each
Catalog Number: LS-G109741-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human CSPP1 (NM_024790), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
CSPP1 | CSPP
Origin Species
Human
NCBI GenBank
NM_024790
SwissProt
Q1MSJ5

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
141.8 kDa
Protein Type
Lysate
Amino Acid Sequence
1MLFPLQVAAV TSSVRDDPLE HCVSPRTRAR SPEICKMADN LDEFIEEQKA RLAEDKAELE
61SDPPYMEMKG KLSAKLSENS KILISMAKEN IPPNSQQTRG SLGIDYGLSL PLGEDYERKK
121HKLKEELRQD YRRYLTQERL KLERNKEYNQ FLRGKEESSE KFRQVEKSTE PKSQRNKKPI
181GQVKPDLTSQ IQTSCENSEG PRKDVLTPSE AYEELLNQRR LEEDRYRQLD DEIELRNRRI
241IKKANEEVGI SNLKHQRFAS KAGIPDRRFH RFNEDRVFDR RYHRPDQDPE VSEEMDERFR
301YESDFDRRLS RVYTNDRMHR NKRGNMPPME HDGDVIEQSN IRISSAENKS APDNETSKSA
361NQDTCSPFAG MLFGGEDREL IQRRKEKYRL ELLEQMAEQQ RNKRREKDLE LRVAASGAQD
421PEKSPDRLKQ FSVAPRHFEE MIPPERPRIA FQTPLPPLSA PSVPPIPSVH PVPSQNEDLR
481SGLSSALGEM VSPRIAPLPP PPLLPPLATN YRTPYDDAYY FYGSRNTFDP SLAYYGSGMM
541GVQPAAYVSA PVTHQLAQPV VNTVGQNELK ITSDQVINSG LIFEDKPKPS KQSLQSYQEA
601LQQQIREREE RRKKEREEKE EYEAKLEAEM RTYNPWGKGG GGAPLRDAKG NLITDLNRMH
661RQNIDAYHNP DARTYEDKRA VVSLDPNLAT SNAENLEDAA NKSSGHMQTQ SSPFARGNVF
721GEPPTELQIK QQELYKNFLR FQIEEKKQRE EAERERLRIA EEKEERRLAE QRARIQQEYE
781EEQEKKREKE EEQRLKNEEH IRLAEERQKE AERKKKEEEE KYNLQLQHYC ERDNLIGEET
841KHMRQPSPIV PALQNKIASK LQRPPSVDSI IRSFIHESSM SRAQSPPVPA RKNQLRAEEE
901KKNVIMELSE MRKQLRSEER RLQERLLHMD SDDEIPIRKK ERNPMDIFDM ARHRLQAPVR
961RQSPKGLDAA TFQNVHDFNE LKDRDSETRV DLKFMYLDPP RDHHTLEIQQ QALLREQQKR
1021LNRIKMQEGA KVDLDAIPSA KVREQRMPRD DTSDFLKNSL LESDSAFIGA YGETYPAIED
1081DVLPPPSQLP SARERRRNKW KGLDIDSSRP NVAPDGLSLK SISSVNVDEL RVRNEERMRR
1141LNEFHNKPIN TDDESSLVDP DDIMKHIGDD GSNSVATEPW LRPGTSETLK RFMAEQLNQE
1201QQQIPGKPGT FTWQGLSTAH G
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1221 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.