Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
CSNK1G3 / CKI-Gamma 3 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G120748-100
Catalog Number: LS-G120748
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human CSNK1G3 (NM_001031812), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Casein kinase 1 gamma 3 | Casein kinase 1 | gamma 3 | CKI-gamma 3 | CSNK1G3L | CSNK1G3 | CK1 gamma 3
- Origin Species
- Human
- NCBI GenBank
- NM_004384
- SwissProt
- Q9Y6M4
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 48.7 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MENKKKDKDK SDDRMARPSG RSGHNTRGTG SSSSGVLMVG PNFRVGKKIG CGNFGELRLG61KNLYTNEYVA IKLEPMKSRA PQLHLEYRFY EQLGSGDGIP QVYYFGPCGK YNAMVLELLG121PSLEDLFDLC DRTFSLKTVL MIAIQLISRM EYVHSKNLIY RDVKPENFLI GRPGNKTQQV181IHIIDFGLAK EYIDPETKKH IPYREHKSLT GTARYMSINT HLGKEQSRRD DLEALGHMFM241YFLRGSLPWQ GLKADTLKER YQKIGDTKRA TPIEVLCENF PEMATYLRYV RRLDFFEKPD301YDYLRKLFTD LFDRKGYMFD YEYDWIGKQL PTPVGAVQQD PALSSNREAH QHRDKMQQSK361NQVVSSTNGE LNTDDPTAGR SNAPITAPTE VEVMDETNCQ KVLNMWCCCF FKRRKRKTIQ421RHKTRTRPLEQKLISEEDLAANDILDYKDDDDKV
423 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.