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CSDE1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

CSDE1 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G114826-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human CSDE1 (NM_007158), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
CSDE1 | N-ras upstream gene protein | KIAA0885 | RP5-1000E10.3 | NRU | Upstream of NRAS | Protein UNR | D1S155E | NRAS-related | UNR
Origin Species
Human
NCBI GenBank
NM_007158
SwissProt
O75534

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
85.6 kDa
Protein Type
Lysate
Amino Acid Sequence
1MSFDPNLLHN NGHNGYPNGT SAALRETGVI EKLLTSYGFI QCSERQARLF FHCSQYNGNL
61QDLKVGDDVE FEVSSDRRTG KPIAVKLVKI KQEILPEERM NGQEVFYLTY TPEDVEGNVQ
121LETGDKINFV IDNNKHTGAV SARNIMLLKK KQARCQGVVC AMKEAFGFIE RGDVVKEIFF
181HYSEFKGDLE TLQPGDDVEF TIKDRNGKEV ATDVRLLPQG TVIFEDISIE HFEGTVTKVI
241PKVPSKNQND PLPGRIKVDF VIPKELPFGD KDTKSKVTLL EGDHVRFNIS TDRRDKLERA
301TNIEVLSNTF QFTNEAREMG VIAAMRDGFG FIKCVDRDVR MFFHFSEILD GNQLHIADEV
361EFTVVPDMLS AQRNHAIRIK KLPKGTVSFH SHSDHRFLGT VEKEATFSNP KTTSPNKGKE
421KEAEDGIIAY DDCGVKLTIA FQAKDVEGST SPQIGDKVEF SISDKQRPGQ QVATCVRLLG
481RNSNSKRLLG YVATLKDNFG FIETANHDKE IFFHYSEFSG DVDSLELGDM VEYSLSKGKG
541NKVSAEKVNK THSVNGITEE ADPTIYSGKV IRPLRSVDPT QTEYQGMIEI VEEGDMKGEV
601YPFGIVGMAN KGDCLQKGES VKFQLCVLGQ NAQTMAYNIT PLRRATVECV KDQFGFINYE
661VGDSKKLFFH VKEVQDGIEL QAGDEVEFSV ILNQRTGKCS ACNVWRVCEG PKAVAAPRPD
721RLVNRLKNIT LDDASAPRLM VLRQPRGPDN SMGFGAERKI RQAGVID
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

767 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.