Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
CSAD / CSD Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G129024-100
Catalog Number: LS-G129024
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human CSAD (NM_015989), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- CSD | Sulfinoalanine decarboxylase | CSAD | PCAP
- Origin Species
- Human
- NCBI GenBank
- NM_015989
- SwissProt
- Q9Y600
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 57.8 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MADSEALPSL AGDPVAVEAL LRAVFGVVVD EAIQKGTSVS QKVCEWKEPE ELKQLLDLEL61RSQGESQKQI LERCRAVIRY SVKTGHPRFF NQLFSGLDPH ALAGRIITES LNTSQYTYEI121APVFVLMEEE VLRKLRALVG WSSGDGIFCP GGSISNMYAV NLARYQRYPD CKQRGLRTLP181PLALFTSKEC HYSIQKGAAF LGLGTDSVRV VKADERGKMV PEDLERQIGM AEAEGAVPFL241VSATSGTTVL GAFDPLEAIA DVCQRHGLWL HVDAAWGGSV LLSQTHRHLL DGIQRADSVA301WNPHKLLAAG LQCSALLLQD TSNLLKRCHG SQASYLFQQD KFYDVALDTG DKVVQCGRRV361DCLKLWLMWK AQGDQGLERR IDQAFVLARY LVEEMKKREG FELVMEPEFV NVCFWFVPPS421LRGKQESPDY HERLSKVAPV LKERMVKEGS MMIGYQPHGT RGNFFRVVVA NSALTCADMD481FLLNELERLG QDLTRTRPLEQKLISEEDLAANDILDYKDDDDKV
493 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.