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CRTAM Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

CRTAM Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G111412-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human CRTAM (NM_019604), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
CD355 | CD355 antigen | CRTAM
Origin Species
Human
NCBI GenBank
NM_019604
SwissProt
O95727

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
44.5 kda
Protein Type
Lysate
Amino Acid Sequence
1MWWRVLSLLA WFPLQEASLT NHTETITVEE GQTLTLKCVT SLRKNSSLQW LTPSGFTIFL
61NEYPVLKNSK YQLLHHSANQ LSITVPNVTL QDEGVYKCLH YSDSVSTKEV KVIVLATPFK
121PILEASVIRK QNGEEHVVLM CSTMRSKPPP QITWLLGNSM EVSGGTLHEF ETDGKKCNTT
181STLIIHTYGK NSTVDCIIRH RGLQGRKLVA PFRFEDLVTD EETASDALER NSLSSQDPQQ
241PTSTVSVTED SSTSEIDKEE KEQTTQDPDL TTEANPQYLG LARKKSGILL LTLVSFLIFI
301LFIIVQLFIM KLRKTHVIWK KENEVSEHTL ESYRSRSNNE ETSSEEKNGQ SSHPMRCMNY
361ITKLYSEGKT KRKENVQHSK LEEKHIQVPE SIV
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

393 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.