Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
CRLF2 / TSLPR Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G110423-100
Catalog Number: LS-G110423
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human CRLF2 (NM_022148), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- CRLF2 | IL-XR | ILXR | p2RY8/CRLF2 fusion | TSLP receptor | TSLPR | CRL2 | CRLF2Y | Cytokine receptor-like 2
- Origin Species
- Human
- NCBI GenBank
- AK026800
- SwissProt
- Q9HC73
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 41.8 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MGRLVLLWGA AVFLLGGWMA LGQGGAEGVQ IQIIYFNLET VQVTWNASKY SRTNLTFHYR61FNGDEAYDQC TNYLLQEGHT SGCLLDAEQR DDILYFSIRN GTHPVFTASR WMVYYLKPSS121PKHVRFSWHQ DAVTVTCSDL SYGDLLYEVQ YRSPFDTEWQ SKQENTCNVT IEGLDAEKCY181SFWVRVKAME DVYGPDTYPS DWSEVTCWQR GEIRDACAET PTPPKPKLSK FILISSLAIL241LMVSLLLLSL WKLWRVRKFL IPSVPDPKSI FPGLFEIHQG NFQEWITDTQ NVAHLHKMAG301AEQESGPEEP LVVQLAKTEA ESPRMLDPQT EEKEASGGSL QLPHQPLQGG DVVTIGGFTF361VMNDRSYVALTRTRPLEQKLISEEDLAANDILDYKDDDDKV
370 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.