Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
CRHR2 / CRF2 Receptor Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G118333-100
Catalog Number: LS-G118333
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Corticotropin Releasing Factor Receptor 2(CRHR2) (NM_001883), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- CRF-R-2 | CRF-R2 | CRF2R | Crh2 receptor | CRHR2 | CRF2 receptor | beta isoform | CRFR2 | CRFR-2 | CRH-R-2 | CRH2R | CRF-RB | CRF2 | CRH-R2 | HM-CRF
- Origin Species
- Human
- NCBI GenBank
- NM_001883
- SwissProt
- Q13324
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 47.5 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MDAALLHSLL EANCSLALAE ELLLDGWGPP LDPEGPYSYC NTTLDQIGTC WPRSAAGALV61ERPCPEYFNG VKYNTTRNAY RECLENGTWA SKINYSQCEP ILDDKQRKYD LHYRIALVVN121YLGHCVSVAA LVAAFLLFLA LRSIRCLRNV IHWNLITTFI LRNVMWFLLQ LVDHEVHESN181EVWCRCITTI FNYFVVTNFF WMFVEGCYLH TAIVMTYSTE RLRKCLFLFI GWCIPFPIIV241AWAIGKLYYE NEQCWFGKEP GDLVDYIYQG PIILVLLINF VFLFNIVRIL MTKLRASTTS301ETIQYRKAVK ATLVLLPLLG ITYMLFFVNP GEDDLSQIMF IYFNSFLQSF QGFFVSVFYC361FFNGEVRSAV RKRWHRWQDH HSLRVPMARA MSIPTSPTRI SFHSIKQTAA VTRTRPLEQKLISEEDLAANDILDYKDDDDKV
411 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.