Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
CREBZF / Zhangfei Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G120641-100
Catalog Number: LS-G120641
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Zhangfei(CREBZF) (NM_001039618), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- CREBZF | SMILE | ZF | Zhangfei
- Origin Species
- Human
- NCBI GenBank
- AF039942
- SwissProt
- Q9NS37
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 37 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MRHSLTKLLA ASGSNFPTRS ESPEPAATCS LPSDLTRAAA GEEETAAAGS PGRKQQFGDE61GELEAGRGSR GGVAVRAPSP EEMEEEAIAS LPGEETEDMD FLSGLELADL LDPRQPDWHL121DPGLSSPGPL SSSGGGSDSG GLWRGDDDDE AAAAEMQRFS DLLQRLLNGI GGCSSSSDSG181SAEKRRRKSP GGGGGGGSGN DNNQAATKSP RKAAAAAARL NRLKKKEYVM GLESRVRGLA241AENQELRAEN RELGKRVQAL QEESRYLRAV LANETGLARL LSRLSGVGLR LTTSLFRDSP301AGDHDYALPV GKQKQDLLEE DDSAGGVCLH VDKDKVSVEF CSACARKASS SLKMSGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV
354 aa + 34 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.