Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
CREB3 / LZIP Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G115350-100
Catalog Number: LS-G115350
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human CREB3 (NM_006368), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Basic leucine zipper protein | CREB-3 | CREB3 | LUMAN | LZIP | Leucin zipper proitein
- Origin Species
- Human
- NCBI GenBank
- NM_006368
- SwissProt
- O43889
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 41.2 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MELELDAGDQ DLLAFLLEES GDLGTAPDEA VRAPLDWALP LSEVPSDWEV DDLLCSLLSP61PASLNILSSS NPCLVHHDHT YSLPRETVSM DLESESCRKE GTQMTPQHME ELAEQEIARL121VLTDEEKSLL EKEGLILPET LPLTKTEEQI LKRVRRKIRN KRSAQESRRK KKVYVGGLES181RVLKYTAQNM ELQNKVQLLE EQNLSLLDQL RKLQAMVIEI SNKTSSSSTC ILVLLVSFCL241LLVPAIYSSD TRGSLPAEHG VLSRQLRALP SEDPYQLELP ALQSEVPKDS THQWLDGSDC301VLQAPGNTSC LLHYMPQAPS AEPPLEWPFP DLFSEPLCRG PILPLQANLT RKGGWLPTGS361PSVILQDRYS GTRTRPLEQKLISEEDLAANDILDYKDDDDKV
371 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.