Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
CRD-BP / ZBP1 / IGF2BP1 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G128132-100
Catalog Number: LS-G128132
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human IGF2BP1 (NM_001160423), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- CRD-BP | CRDBP | IGF2BP1 | IMP1 | IGF2 mRNA-binding protein 1 | IMP-1 | ZBP-1 | Zip code-binding protein 1 | Zipcode-binding protein 1 | VICKZ family member 1 | IGF-II mRNA-binding protein 1 | VICKZ1
- Origin Species
- Human
- NCBI GenBank
- NM_006546
- SwissProt
- Q9NZI8
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 48.4 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MNKLYIGNLN ESVTPADLEK VFAEHKISYS GQFLVKSGYA FVDCPDEHWA MKAIETFSGK61VELQGKRLEI EHSVPKKQRS RKIQIRNIPP QLRWEVLDSL LAQYGTVENC EQVNTESETA121VVNVTYSNRE QTRQADEVPL KILAHNNFVG RLIGKEGRNL KKVEQDTETK ITISSLQDLT181LYNPERTITV KGAIENCCRA EQEIMKKVRE AYENDVAAMS LQSHLIPGLN LAAVGLFPAS241SSAVPPPPSS VTGAAPYSSF MQAPEQEMVQ VFIPAQAVGA IIGKKGQHIK QLSRFASASI301KIAPPETPDS KVRMVIITGP PEAQFKAQGR IYGKLKEENF FGPKEEVKLE THIRVPASAA361GRVIGKGGKT VNELQNLTAA EVVVPRDQTP DENDQVIVKI IGHFYASQMA QRKIRDILAQ421VKQQHQKGQS NQAQARRKTRTRPLEQKLISEEDLAANDILDYKDDDDKV
438 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.