Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
CPXM1 / CPXM Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G130140-100
Catalog Number: LS-G130140
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human CPXM(CPXM1) (NM_001184699), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- CPX-1 | CPXM | CPXM1 | Probable carboxypeptidase X1 | CPX1 | Metallocarboxypeptidase CPX-1
- Origin Species
- Human
- NCBI GenBank
- NM_019609
- SwissProt
- Q96SM3
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 73.9 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MWGLLLALAA FAPAVGPALG APRNSVLGLA QPGTTKVPGS TPALHSSPAQ PPAETANGTS61EQHVRIRVIK KKKVIMKKRK KLTLTRPTPL VTAGPLVTPT PAGTLDPAEK QETGCPPLGL121ESLRVSDSRL EASSSQSFGL GPHRGRLNIQ SGLEDGDLYD GAWCAEEQDA DPWFQVDAGH181PTRFSGVITQ GRNSVWRYDW VTSYKVQFSN DSRTWWGSRN HSSGMDAVFP ANSDPETPVL241NLLPEPQVAR FIRLLPQTWL QGGAPCLRAE ILACPVSDPN DLFLEAPASG SSDPLDFQHH301NYKAMRKLMK QVQEQCPNIT RIYSIGKSYQ GLKLYVMEMS DKPGEHELGE PEVRYVAGMH361GNEALGRELL LLLMQFLCHE FLRGNPRVTR LLSEMRIHLL PSMNPDGYEI AYHRGSELVG421WAEGRWNNQS IDLNHNFADL NTPLWEAQDD GKVPHIVPNH HLPLPTYYTL PNATVAPETR481AVIKWMKRIP FVLSANLHGG MNDFSYLHTN CFEVTVELSC DKFPHENELP QEWENNKDAL541LTYLEQVRMG IAGVVRDKDT ELGIADAVIA VDGINHDVTT AWGGDYWRLL TPGDYMVTAS601AEGYHSVTRN CRVTFEEGPF PCNFVLTKTP KQRLRELLAA GAKVPPDLRR RLERLRGQKDTRTRPLEQKLISEEDLAANDILDYKDDDDKV
660 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.