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CPSF3L Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

CPSF3L Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G112168-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human INT11(CPSF3L) (NM_017871), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
INT11 | Integrator complex subunit 11 | INTS11 | RC-68 | CPSF3-like protein | CPSF3L | CPSF73L | RC68
Origin Species
Human
NCBI GenBank
NM_032179
SwissProt
Q5TA45

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
67.5 kDa
Protein Type
Lysate
Amino Acid Sequence
1MPEIRVTPLG AGQDVGRSCI LVSIAGKNVM LDCGMHMGFN DDRRFPDFSY ITQNGRLTDF
61LDCVIISHFH LDHCGALPYF SEMVGYDGPI YMTHPTQAIC PILLEDYRKI AVDKKGEANF
121FTSQMIKDCM KKVVAVHLHQ TVQVDDELEI KAYYAGHVLG AAMFQIKVGS ESVVYTGDYN
181MTPDRHLGAA WIDKCRPNLL ITESTYATTI RDSKRCRERD FLKKVHETVE RGGKVLIPVF
241ALGRAQELCI LLETFWERMN LKVPIYFSTG LTEKANHYYK LFIPWTNQKI RKTFVQRNMF
301EFKHIKAFDR AFADNPGPMV VFATPGMLHA GQSLQIFRKW AGNEKNMVIM PGYCVQGTVG
361HKILSGQRKL EMEGRQVLEV KMQVEYMSFS AHADAKGIMQ LVGQAEPESV LLVHGEAKKM
421EFLKQKIEQE LRVNCYMPAN GETVTLPTSP SIPVGISLGL LKREMAQGLL PEAKKPRLLH
481GTLIMKDSNF RLVSSEQALK ELGLAEHQLR FTCRVHLHDT RKEQETALRV YSHLKSVLKD
541HCVQHLPDGS VTVESVLLQA AAPSEDPGTK VLLVSWTYQD EELGSFLTSL LKKGLPQAPS
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

600 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.