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CPNE7 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

CPNE7 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G105727-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human CPNE7 (NM_153636), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Copine 7 | Copine VII | Copine-7 | CPNE7
Origin Species
Human
NCBI GenBank
NM_014427
SwissProt
Q9UBL6

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
61.8 kDa
Protein Type
Lysate
Amino Acid Sequence
1MSAGSERGAA ATPGGLPAPC ASKVELRLSC RHLLDRDPLT KSDPSVALLQ QAQGQWVQVG
61RTEVVRSSLH PVFSKVFTVD YYFEEVQRLR FEVYDTHGPS GFSCQEDDFL GGMECTLGQI
121VAQKKVTRPL LLKFGRNAGK STITVIAEDI SGNNGYVELS FRARKLDDKD LFSKSDPFLE
181LYRVNDDQGL QLVYRTEVVK NNLNPVWEAF KVSLSSLCSC EETRPLKCLV WDYDSRGKHD
241FIGEFSTTFE EMQKAFEEGQ AQWDCVNPKY KQKRRSYKNS GVVVLADLKF HRVYSFLDYI
301MGGCQIHFTV AIDFTASNGD PRNSCSLHYI NPYQPNEYLK ALVSVGEICQ DYDSDKRFSA
361LGFGARIPPK YEVSHDFAIN FNPEDDECEG IQGVVEAYQN CLPRVQLYGP TNVAPIISKV
421ARVAAAEEST GKASQYYILL ILTDGVVTDM ADTREAIVRA SRLPMSIIIV GVGNADFTDM
481QVLDGDDGVL RSPRGEPALR DIVQFVPFRE LKNASPAALA KCVLAEVPKQ VVEYYSHRGL
541PPRSLGVPAG EASPGCTP
SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV

558 aa + 34 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.