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CPNE4 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

CPNE4 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G102213-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human CPNE4 (NM_130808), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Copine IV | Copine-4 | CPNE4 | Copine 8 | Copine-8 | COPN4 | CPN4
Origin Species
Human
SwissProt
Q96A23

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
62.2 kDa
Protein Type
Lysate
Amino Acid Sequence
1MKKMSNIYES AANTLGIFNS PCLTKVELRV ACKGISDRDA LSKPDPCVIL KMQSHGQWFE
61VDRTEVIRTC INPVYSKLFT VDFYFEEVQR LRFEVHDISS NHNGLKEADF LGGMECTLGQ
121IVSQRKLSKS LLKHGNTAGK SSITVIAEEL SGNDDYVELA FNARKLDDKD FFSKSDPFLE
181IFRMNDDATQ QLVHRTEVVM NNLSPAWKSF KVSVNSLCSG DPDRRLKCIV WDWDSNGKHD
241FIGEFTSTFK EMRGAMEGKQ VQWECINPKY KAKKKNYKNS GTVILNLCKI HKMHSFLDYI
301MGGCQIQFTV AIDFTASNGD PRNSCSLHYI HPYQPNEYLK ALVAVGEICQ DYDSDKMFPA
361FGFGARIPPE YTVSHDFAIN FNEDNPECAG IQGVVEAYQS CLPKLQLYGP TNIAPIIQKV
421AKSASEETNT KEASQYFILL ILTDGVITDM ADTREAIVHA SHLPMSVIIV GVGNADFSDM
481QMLDGDDGIL RSPKGEPVLR DIVQFVPFRN FKHASPAALA KSVLAEVPNQ VVDYYNGKGI
541KPKCSSEMYE SSRTLAP
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

557 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.