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CPEB4 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

CPEB4 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G101955-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human CPEB4 (NM_030627), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
CPE-binding protein 4 | CPE-BP4 | CPEB4 | HCPEB-4 | KIAA1673
Origin Species
Human
SwissProt
Q17RY0

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
80 kDa
Protein Type
Lysate
Amino Acid Sequence
1MGDYGFGVLV QSNTGNKSAF PVRFHPHLQP PHHHQNATPS PAAFINNNTA ANGSSAGSAW
61LFPAPATHNI QDEILGSEKA KSQQQEQQDP LEKQQLSPSP GQEAGILPET EKAKSEENQG
121DNSSENGNGK EKIRIESPVL TGFDYQEATG LGTSTQPLTS SASSLTGFSN WSAAIAPSSS
181TIINEDASFF HQGGVPAASA NNGALLFQNF PHHVSPGFGG SFSPQIGPLS QHHPHHPHFQ
241HHHSQHQQQR RSPASPHPPP FTHRNAAFNQ LPHLANNLNK PPSPWSSYQS PSPTPSSSWS
301PGGGGYGGWG GSQGRDHRRG LNGGITPLNS ISPLKKNFAS NHIQLQKYAR PSSAFAPKSW
361MEDSLNRADN IFPFPDRPRT FDMHSLESSL IDIMRAENDT IKGRLNYSYP GSDSSLLINA
421RTYGRRRGQS SLFPMEDGFL DDGRGDQPLH SGLGSPHCFS HQNGERVERY SRKVFVGGLP
481PDIDEDEITA SFRRFGPLIV DWPHKAESKS YFPPKGYAFL LFQDESSVQA LIDACIEEDG
541KLYLCVSSPT IKDKPVQIRP WNLSDSDFVM DGSQPLDPRK TIFVGGVPRP LRAVELAMIM
601DRLYGGVCYA GIDTDPELKY PKGAGRVAFS NQQSYIAAIS ARFVQLQHGE IDKRVEVKPY
661VLDDQLCDEC QGARCGGKFA PFFCANVTCL QYYCEYCWAA IHSRAGREFH KPLVKEGGDR
721PRHISFRWN
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

729 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.