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CPEB3 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

CPEB3 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G130158-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human CPEB3 (NM_001178137), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
CPE-binding protein 3 | HCPEB-3 | KIAA0940 | CPE-BP3 | CPEB3
Origin Species
Human
NCBI GenBank
NM_014912
SwissProt
Q8NE35

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
74.9 kDa
Protein Type
Lysate
Amino Acid Sequence
1MQDDLLMDKS KTQPQPQQQQ RQQQQPQPES SVSEAPSTPL SSETPKPEEN SAVPALSPAA
61APPAPNGPDK MQMESPLLPG LSFHQPPQQP PPPQEPAAPG ASLSPSFGST WSTGTTNAVE
121DSFFQGITPV NGTMLFQNFP HHVNPVFGGT FSPQIGLAQT QHHQQPPPPA PAPQPAQPAQ
181PPQAQPPQQR RSPASPSQAP YAQRSAAAAY GHQPIMTSKP SSSSAVAAAA AAAAASSASS
241SWNTHQSVNA AWSAPSNPWG GLQAGRDPRR AVGVGVGVGV GVPSPLNPIS PLKKPFSSNV
301IAPPKFPRAA PLTSKSWMED NAFRTDNGNN LLPFQDRSRP YDTFNLHSLE NSLMDMIRTD
361HEPLKGRMGI NFHHPGTDNI MALNSRSSLF PFEDAFLDDS HGDQALSSGL SSPTRCQNGE
421RVERYSRKVF VGGLPPDIDE DEITASFRRF GPLVVDWPHK AESKSYFPPK GYAFLLFQEE
481SSVQALIDAC LEEDGKLYLC VSSPTIKDKP VQIRPWNLSD SDFVMDGSQP LDPRKTIFVG
541GVPRPLRAVE LAMIMDRLYG GVCYAGIDTD PELKYPKGAG RVAFSNQQSY IAAISARFVQ
601LQHNDIDKRV EVKPYVLDDQ MCDECQGTRC GGKFAPFFCA NVTCLQYYCE YCWASIHSRA
661GREFHKPLVK EGGDRPRHVP FRWS
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

684 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.