Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
CPB2 / TAFI Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G112680-100
Catalog Number: LS-G112680
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Carboxypeptidase B2(CPB2) (NM_016413), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Carboxypeptidase R | Carboxypeptidase U | Carboxypeptidase B-like | Carboxypeptidase B2 (plasma) | TAFI | Plasma carboxypeptidase B | Carboxypeptidase B2 | CPB2 | CPU | PCPB
- Origin Species
- Human
- NCBI GenBank
- M75106
- SwissProt
- Q96IY4
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 40.7 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MKLCSLAVLV PIVLFCEQHV FAFQSGQVLA ALPRTSRQVQ VLQNLTTTYE IVLWQPVTAD61LIVKKKQVHF FVNASDVDNV KAHLNVSGIP CSVLLADVED LIQQQISNDT VSPRASASYY121EQYHSLNEIY SWIEFITERH PDMLTKIHIG SSFEKYPLYV LKVSGKEQAA KNAIWIDCGI181HAREWISPAF CLWFIGHNRM WRKNRSFYAN NHCIGTDLNR NFASKHWCEE GASSSSCSET241YCGLYPESEP EVKAVASFLR RNINQIKAYI SMHSYSQHIV FPYSYTRSKS KDHEELSLVA301SEAVRAIEKI SKNTRYTHGH GSETLYLAPG GGDDWIYDLG IKYSFTSNPP VEKLLPLSLKTRTRPLEQKLISEEDLAANDILDYKDDDDKV
360 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.