Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
CPB / Carboxypeptidase B Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G118352-100
Catalog Number: LS-G118352
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Carboxypeptidase B(CPB1) (NM_001871), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Carboxypeptidase B | Carboxypeptidase B1 (tissue) | CPB | CPB1 | Pancreatic carboxypeptidase B | PASP | Procarboxypeptidase B | Protaminase | Pancreas-specific protein | PCPB | Tissue carboxypeptidase B
- Origin Species
- Human
- NCBI GenBank
- NM_001871
- SwissProt
- P15086
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 47.37 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MLALLVLVTV ALASAHHGGE HFEGEKVFRV NVEDENHINI IRELASTTQI DFWKPDSVTQ61IKPHSTVDFR VKAEDTVTVE NVLKQNELQY KVLISNLRNV VEAQFDSRVR ATGHSYEKYN121KWETIEAWTQ QVATENPALI SRSVIGTTFE GRAIYLLKVG KAGQNKPAIF MDCGFHAREW181ISPAFCQWFV REAVRTYGRE IQVTELLDKL DFYVLPVLNI DGYIYTWTKS RFWRKTRSTH241TGSSCIGTDP NRNFDAGWCE IGASRNPCDE TYCGPAAESE KETKALADFI RNKLSSIKAY301LTIHSYSQMM IYPYSYAYKL GENNAELNAL AKATVKELAS LHGTKYTYGP GATTIYPAAG361GSDDWAYDQG IRYSFTFELR DTGRYGFLLP ESQIRATCEE TFLAIKYVAS YVLEHLYTRTRPLEQKLISEEDLAANDILDYKDDDDKV
417 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.