Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
CPA1 / Carboxypeptidase A Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G118349-100
Catalog Number: LS-G118349
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Carboxypeptidase A(CPA1) (NM_001868), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- CPA | Carboxypeptidase A1 | CPA1 | Pancreatic carboxypeptidase A | Carboxypeptidase A
- Origin Species
- Human
- NCBI GenBank
- NM_001868
- SwissProt
- P15085
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 47.16 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MRGLLVLSVL LGAVFGKEDF VGHQVLRISV ADEAQVQKVK ELEDLEHLQL DFWRGPAHPG61SPIDVRVPFP SIQAVKIFLE SHGISYETMI EDVQSLLDEE QEQMFAFRSR ARSTDTFNYA121TYHTLEEIYD FLDLLVAENP HLVSKIQIGN TYEGRPIYVL KFSTGGSKRP AIWIDTGIHS181REWVTQASGV WFAKKITQDY GQDAAFTAIL DTLDIFLEIV TNPDGFAFTH STNRMWRKTR241SHTAGSLCIG VDPNRNWDAG FGLSGASSNP CSETYRGKFA NSEVEVKSIV DFVKDHGNIK301AFISIHSYSQ LLMYPYGYKT EPVPDQDELD QLSKAAVTAL ASLYGTKFNY GSIIKAIYQA361SGSTIDWTYS QGIKYSFTFE LRDTGRYGFL LPASQIIPTA KETWLALLTI MEHTLNHPYT421RTRPLEQKLI SEEDLAANDI LDYKDDDDKV
450 aa
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.