Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
COT / CROT Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G110746-100
Catalog Number: LS-G110746
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human CROT (NM_021151), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- COT | Carnitine octanoyltransferase | CROT
- Origin Species
- Human
- NCBI GenBank
- NM_021151
- SwissProt
- Q9UKG9
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 70 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MENQLAKSTE ERTFQYQDSL PSLPVPSLEE SLKKYLESVK PFANQEEYKK TEEIVQKFQS61GIGEKLHQKL LERAKGKRNW LEEWWLNVAY LDVRIPSQLN VNFAGPAAHF EHYWPPKEGT121QLERGSITLW HNLNYWQLLR KEKVPVHKVG NTPLDMNQFR MLFSTCKVPG ITRDSIMNYF181RTESEGRSPN HIVVLCRGRA FVFDVIHEGC LVTPPELLRQ LTYIHKKCHS EPDGPGIAAL241TSEERTRWAK AREYLIGLDP ENLALLEKIQ SSLLVYSMED SSPHVTPEDY SEIIAAILIG301DPTVRWGDKS YNLISFSNGV FGCNCDHAPF DAMIMVNISY YVDEKIFQNE GRWKGSEKVR361DIPLPEELIF IVDEKVLNDI NQAKAQYLRE ASDLQIAAYA FTSFGKKLTK NKMLHPDTFI421QLALQLAYYR LHGHPGCCYE TAMTRHFYHG RTETMRSCTV EAVRWCQSMQ DPSVNLRERQ481QKMLQAFAKH NKMMKDCSAG KGFDRHLLGL LLIAKEEGLP VPELFTDPLF SKSGGGGNFV541LSTSLVGYLR VQGVVVPMVH NGYGFFYHIR DDRFVVACSA WKSCPETDAE KLVQLTFCAF601HDMIQLMNST HLTRTRPLEQKLISEEDLAANDILDYKDDDDKV
612 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.