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Coronavirus SARS-CoV-2 Orf1ab Polyprotein (Recombinant)

Coronavirus SARS-CoV-2 Orf1ab Polyprotein (Recombinant)

ProteinLSBio Guarantee
Expression SystemEscherichia coli (Prokaryotic)
Format:Liquid
$350.00each
Catalog Number: LS-G145879-10

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Overview

Description
Replication of severe acute respiratory syndrome (SARS) coronavirus (SARS-CoV) requires proteolytic processing of the replicase polyprotein by two viral cysteine proteases, a chymotrypsin-like protease (3CLpro) and a papain-like protease (PLpro). These proteases are important targets for development of antiviral drugs that would inhibit viral replication and reduce mortality associated with outbreaks of SARS-CoV. PLpro is a cysteine protease located within the non-structural protein 3 (NS3) section of the viral polypeptide. PLPro activity is required to process the viral polyprotein into functional, mature subunits; specifically, PLPro cleaves a site at the amino-terminus of the viral replicase region. In addition to its role in viral protein maturation, PLPro possesses a deubiquitinating and deISGylating activity. In vivo, this protease antagonizes innate immunity by inhibiting IRF3-induced production of type I interferons.
Category
Proteins & Peptides > Proteins > Recombinant Proteins
Origin Species
Virus / Viral Antigen

Protein

Expression System
Escherichia coli (Prokaryotic)
Molecular Weight
35.8 kDa
Protein Type
Recombinant
Amino Acid Sequence
1Glu1564-Ly s1878

15 aa

Format

Purity
>90%
Conjugate/Label
Unconjugated
Endotoxin Level
< 1.0 EU/µg
Format
Liquid
Formulation
Sterile solution in 20 mM Tris-HCl, pH 7.5, 10 mM Beta-ME, 20% Glycerol
Concentration
1.5 mg/mL
Purity Measurement Method
SDS-PAGE (Coomassie Stain)

Storage & Handling

Storage Conditions
Store at less than -20°C for up to 6 months. Avoid freeze-thaw cycles.

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