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CORO2A Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

CORO2A Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G102142-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human CORO2A (NM_052820), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
CORO2A | Coronin-2A | Coronin-like protein B | CLIPINB | Coronin 2A | IR10 | WD repeat-containing protein 2 | WD protein IR10 | WD-repeat protein 2 | WDR2
Origin Species
Human
NCBI GenBank
NM_003389

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
59.6 kDa
Protein Type
Lysate
Amino Acid Sequence
1MSWHPQYRSS KFRHVFGKPA SKENCYDSVP ITRSVHDNHF CAVNPHFIAV VTECAGGGAF
61LVIPLHQTGK LDPHYPKVCG HRGNVLDVKW NPFDDFEIAS CSEDATIKIW SIPKQLLTRN
121LTAYRKELVG HARRVGLVEW HPTAANILFS AGYDYKVMIW NLDTKESVIT SPMSTISCHQ
181DVILSMSFNT NGSLLATTCK DRKIRVIDPR AGTVLQEASY KGHRASKVLF LGNLKKLMST
241GTSRWNNRQV ALWDQDNLSV PLMEEDLDGS SGVLFPFYDA DTSMLYVVGK GDGNIRYYEV
301SADKPHLSYL TEYRSYNPQK GIGVMPKRGL DVSSCEIFRF YKLITTKSLI EPISMIVPRR
361SESYQEDIYP PTAGAQPSLT AQEWLSGMNR DPILVSLRPG SELLRPHPLP AERPIFNSMA
421PASPRLLNQT EKLAAEDGWR SSSLLEEKMP RWAAEHRLEE KKTWLTNGFD VFECPPPKTE
481NELLQMFYRQ QEEIRRLREL LTQREVQAKQ LELEIKNLRM GSEQL
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

525 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.