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CORO1C Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

CORO1C Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all CORO1C antibodies →
$504.00each
Catalog Number: LS-G114024-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Coronin 3(CORO1C) (NM_014325), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
CRNN4 | CORO1C | Coronin 1C | Coronin-3 | Coronin-1C | HCRNN4
Origin Species
Human
NCBI GenBank
NM_014325
SwissProt
Q9ULV4

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
53.1 kda
Protein Type
Lysate
Amino Acid Sequence
1MRRVVRQSKF RHVFGQAVKN DQCYDDIRVS RVTWDSSFCA VNPRFVAIII EASGGGAFLV
61LPLHKTGRID KSYPTVCGHT GPVLDIDWCP HNDQVIASGS EDCTVMVWQI PENGLTLSLT
121EPVVILEGHS KRVGIVAWHP TARNVLLSAG CDNAIIIWNV GTGEALINLD DMHSDMIYNV
181SWNRNGSLIC TASKDKKVRV IDPRKQEIVA EKEKAHEGAR PMRAIFLADG NVFTTGFSRM
241SERQLALWNP KNMQEPIALH EMDTSNGVLL PFYDPDTSII YLCGKGDSSI RYFEITDESP
301YVHYLNTFSS KEPQRGMGYM PKRGLDVNKC EIARFFKLHE RKCEPIIMTV PRKSDLFQDD
361LYPDTAGPEA ALEAEEWFEG KNADPILISL KHGYIPGKNR DLKVVKKNIL DSKPTANKKC
421DLISIPKKTT DTASVQNEAK LDEILKEIKS IKDTICNQDE RISKLEQQMA KIAATRTRPL
481EQKLISEEDL AANDILDYKD DDDKV

505 aa

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.