Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Corneodesmosin / CDSN Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G118694-100
Catalog Number: LS-G118694
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Corneodesmosin(CDSN) (NM_001264), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- D6S586E | HTSS1 | HTSS | S protein | CDSN | Corneodesmosin | PSS
- Origin Species
- Human
- NCBI GenBank
- NM_001264
- SwissProt
- Q15517
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 51.72 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MGSSRAPWMG RVGGHGMLAL LLAGLLLPGT LAKSIGTFSD PCKDPTRITS PNDPCLTGKG61DSSGFSSYSG SSSSGSSISS ARSSGGGSSG SSSGSSIAQG GSAGSFKPGT GYSQVSYSSG121SGSSLQGASG SSQLGSSSSH SGSSGSHSGS SSHSSSSSSF QFSSSSFQVG NGSALPTNDN181SYRGILNPSQ PGQSSSSSQT FGVSSSGQSV SSNQRPCSSD IPDSPCSGGP IVSHSGPYIP241SSHSVSGGQR PVVVVVDQHG SGAPGVVQGP PCSNGGLPGK PCPPITSVDK SYGGYEVVGG301SSDSYLVPGM TYSKGKIYPV GYFTKENPVK GSPGVPSFAA GPPISEGKYF SSNPIIPSQS361AASSAIAFQP VGTGGVQLCG GGSTGSKGPC SPSSSRVPSS SSISSSSGLP YHPCGSASQS421PCSPPGTGSF SSSSSSQSSG KIILQPCGSK SSSSGHPCMS VSSLTLTGGP DGSPHPDPSA481GAKPCGSSSA GKIPCRSIRD ILAQVKPLGP QLADPEVFLP QGELLDSPSG PSGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV
531 aa + 34 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.