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COQ6 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

COQ6 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G104302-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human COQ6 (NM_182480), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Coenzyme Q10 monooxygenase 6 | Coenzyme Q6 homolog (yeast) | CGI-10 | COQ6 | CGI10 | COQ10D6
Origin Species
Human
NCBI GenBank
BC014181
SwissProt
Q9Y2Z9

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
43 kDa
Protein Type
Lysate
Amino Acid Sequence
1MRGQGPPLSS FGVWLASRAA SDPSRPRRQG YDIHFHDKKI LLLEAGPKKV LEKLSETYSN
61RVSSISPGSA TLLSSFGAWD HICNMRYRAF RRMQVWDACS EALIMFDKDN LDDMGYIVEN
121DVIMHALTKQ LEAVSDRVTV LYRSKAIRYT WPCPFPMADS SPWVHITLGD GSTFQTKLLI
181GADGHNSGVR QAVGIQNVSW NYDQSAVVAT LHLSEATENN VAWQRFLPSG PIALLPLSDT
241LSSLVWSTSH EHAAELVSMD EEKFVDAVNS AFWSDADHTD FIDTAGAMLQ YAVSLLKPTK
301VSARQLPPSV ARVDAKSRVL FPLGLGHAAE YVRPRVALIG DAAHRVHPLA GQGVNMGFGD
361ISSLAHHLST AAFNGKDLGS VSHLTGYETE RQRHNTALLA ATDLLKRLYS TSASPLVLLR
421TWGLQATNAV SPLKEQIMAF ASK
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

443 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.