Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
COPS3 / CSN3 Protein (Over-Expression Lysate Myc-DDK (Flag))
ProteinLSBio Guarantee
| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G130932-100
Catalog Number: LS-G130932
For bulk orders or custom formulations:
Request a QuoteOverview
- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human COPS3 (NM_001199125), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- COP9 complex subunit 3 | COPS3 | SGN3 | Signalosome subunit 3
- Origin Species
- Human
- NCBI GenBank
- NM_003653
- SwissProt
- Q9UNS2
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 46.2 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MTQLCELINK SGELLAKNLS HLDTVLGALD VQEHSLGVLA VLFVKFSMPS VPDFETLFSQ61VQLFISTCNG EHIRYATDTF AGLCHQLTNA LVERKQPLRG IGILKQAIDK MQMNTNQLTS121IHADLCQLCL LAKCFKPALP YLDVDMMDIC KENGAYDAKH FLCYYYYGGM IYTGLKNFER181ALYFYEQAIT TPAMAVSHIM LESYKKYILV SLILLGKVQQ LPKYTSQIVG RFIKPLSNAY241HELAQVYSTN NPSELRNLVN KHSETFTRDN NMGLVKQCLS SLYKKNIQRL TKTFLTLSLQ301DMASRVQLSG PQEAEKYVLH MIEDGEIFAS INQKDGMVSF HDNPEKYNNP AMLHNIDQEM361LKCIELDERL KAMDQEITVN PQFVQKSMGS QEDDSGNKPS SYSTRTRPLEQKLISEEDLAANDILDYKDDDDKV
403 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.