Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
COPS2 / TRIP15 / ALIEN Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G126229-100
Catalog Number: LS-G126229
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human TRIP15(COPS2) (NM_001143887), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Alien homolog | ALIEN | SGN2 | TRIP15 | TR-interacting protein 15 | COPS2 | Signalosome subunit 2 | TRIP-15
- Origin Species
- Human
- NCBI GenBank
- NM_004236
- SwissProt
- P61201
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 52.2 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MSDMEDDFMC DDEEDYDLEY SEDSNSEPNV DLENQYYNSK ALKEDDPKAA LSSFQKVLEL61EGEKGEWGFK ALKQMIKINF KLTNFPEMMN RYKQLLTYIR SAVTRNYSEK SINSILDYIS121TSKQNSDFLC QMDLLQEFYE TTLEALKDAK NDRLWFKTNT KLGKLYLERE EYGKLQKILR181QLHQSCQTDD GEDDLKKGTQ LLEIYALEIQ MYTAQKNNKK LKALYEQSLH IKSAIPHPLI241MGVIRECGGK MHLREGEFEK AHTDFFEAFK NYDESGSPRR TTCLKYLVLA NMLMKSGINP301FDSQEAKPYK NDPEILAMTN LVSAYQNNDI TEFEKILKTN HSNIMDDPFI REHIEELLRN361IRTQVLIKLI KPYTRIHIPF ISKELNIDVA DVESLLVQCI LDNTIHGRID QVNQLLELDH421QKRGGARYTA LDKWTNQLNS LNQAVVSKLATRTRPLEQKLISEEDLAANDILDYKDDDDKV
450 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.