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COLQ Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

COLQ Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all COLQ antibodies →
$725.00each
Catalog Number: LS-G107834-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human COLQ (NM_080539), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
AChE Q subunit | COLQ | EAD
Origin Species
Human
NCBI GenBank
NM_005677
SwissProt
Q9Y215

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
41.7 kDa
Protein Type
Lysate
Amino Acid Sequence
1MVVLNPMTLG IYLQLFFLSI VSQPTFINSV LPISAALPSL DQKKRGGHKA CCLLTPPPPP
61LFPPPFFRGG RSPGPPGLPG KTGPKGEKGE LGRPGRKGRP GPPGVPGMPG PIGWPGPEGP
121RGEKGDLGMM GLPGSRGPMG SKGYPGSRGE KGSRGEKGDL GPKGEKGFPG FPGMLGQKGE
181MGPKGEPGIA GHRGPTGRPG KRGKQGQKGD SGVMGPPGKP GPSGQPGRPG PPGPPPAGQL
241IMGPKGERGF PGPPGRCLCG PTMNVNNPSY GESVYGPSSP RVPVIFVVNN QEELERLNTQ
301NAIAFRRDQR SLYFKDSLGW LPIQLTPFYP VDYTADQHGT CGDGLLQPGE ECDDGNSDVG
361DDCIRCHRAY CGDGHRHEGV EDCDGSDFGY LTCETYLPGS YGDLQCTQYC YIDSTPCRYF
421T
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

421 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.