Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
COL6A2 / Collagen VI Alpha 2 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G107947-100
Catalog Number: LS-G107947
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Collagen VI(COL6A2) (NM_058174), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Collagen alpha-2(VI) chain | Collagen | type VI | alpha 2 | COL6A2 | Collagen alpha 2(vi) | PP3610
- Origin Species
- Human
- NCBI GenBank
- AL096746
- SwissProt
- P12110
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 95.3 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MLQGTCSVLL LWGILGAIQA QQQEVISPDT TERNNNCPEK TDCPIHVYFV LDTSESVTMQ61SPTDILLFHM KQFVPQFISQ LQNEFYLDQV ALSWRYGGLH FSDQVEVFSP PGSDRASFIK121NLQGISSFRR GTFTDCALAN MTEQIRQDRS KGTVHFAVVI TDGHVTGSPC GGIKLQAERA181REEGIRLFAV APNQNLKEQG LRDIASTPHE LYRNDYATML PDSTEINQDT INRIIKVMKH241EAYGECYKVS CLEIPGPSGP KGYRGQKGAK GNMGEPGEPG QKGRQGDPGI EGPIGFPGPK301GVPGFKGEKG EFGADGRKGA PGLAGKNGTD GQKGKLGRIG PPGCKGDPGN RGPDGYPGEA361GSPGERGDQG GKGDPGRPGR RGPPGEIGAK GSKGYQGNNG APGSPGVKGA KGGPGPRGPK421GEPGRRGDPG TKGSPGSDGP KGEKGDPGPE GPRGLAGEVG NKGAKGDRGL PGPRGPQGAL481GEPGKQGSRG DPGDAGPRGD SGQPGPKGDP GRPGFSYPGP RGAPGEKGEP GPRGPEGGRG541DFGLKGEPGR KGEKGEPADP GPPGEPGPRG PRGVPGPEGE PGPPGDPGLT ECDVMTYVRE601TCGCCDCEKR CGALDVVFVI DSSESIGYTN FTLEKNFVIN VVNRLGAIAK DPKSETGTRV661GVVQYSHEGT FEAIQLDDEH IDSLSSFKEA VKNLEWIAGG TWTPSALKFA YDRLIKESRR721QKTRVFAVVI TDGRHDPRDD DLNLRALCDR DVTVTAIGIG DMFHEKHESE NLYSIACDKP781QQVRNMTLFS DLVAEKFIDD MEDVLCPDPQ IVCPDLPCQT DAPWPGGEPP VTFLRTEEGP841DATFPRTIPL IQQLLNATEL TQDPAAYSQL VAVLVYTAER AKFATGVERQ DWMELFIDTF901KLVHRDIVGD PETALALCSGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV
918 aa + 34 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.