Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
COL4A3BP / CERT Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G101992-100
Catalog Number: LS-G101992
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human COL4A3BP (NM_031361), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Ceramide transfer protein | Ceramide transporter | CERT | COL4A3BP | CERTL | Lipid-transfer protein CERTL | HCERT | STARD11 | GPBP
- Origin Species
- Human
- NCBI GenBank
- NM_005713
- SwissProt
- Q9Y5P4
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 67.8 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MSDNQSWNSS GSEEDPETES GPPVERCGVL SKWTNYIHGW QDRWVVLKNN ALSYYKSEDE61TEYGCRGSIC LSKAVITPHD FDECRFDISV NDSVWYLRAQ DPDHRQQWID AIEQHKTESG121YGSESSLRRH GSMVSLVSGA SGYSATSTSS FKKGHSLREK LAEMETFRDI LCRQVDTLQK181YFDACADAVS KDELQRDKVV EDDEDDFPTT RSDGDFLHST NGNKEKLFPH VTPKGINGID241FKGEAITFKA TTAGILATLS HCIELMVKRE DSWQKRLDKE TEKKRRTEEA YKNAMTELKK301KSHFGGPDYE EGPNSLINEE EFFDAVEAAL DRQDKIEEQS QSEKVRLHWP TSLPSGDAFS361SVGTHRFVQK VEEMVQNHMT YSLQDVGGDA NWQLVVEEGE MKVYRREVEE NGIVLDPLKA421THAVKGVTGH EVCNYFWNVD VRNDWETTIE NFHVVETLAD NAIIIYQTHK RVWPASQRDV481LYLSVIRKIP ALTENDPETW IVCNFSVDHD SAPLNNRCVR AKINVAMICQ TLVSPPEGNQ541EISRDNILCK ITYVANVNPG GWAPASVLRA VAKREYPKFL KRFTSYVQEK TAGKPILFSG601PTRTRRLEQK LISEEDLAAN DILDYKDDDD KV
632 aa
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.