Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
COL26A1 / EMID2 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G107502-100
Catalog Number: LS-G107502
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human EMID2(COL26A1) (NM_133457), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- COL26A1 | Collagen alpha-1(XXVI) chain | Collagen | type XXVI | alpha 1 | EMI6 | EMI domain containing 2 | EMID2 | KIAA1299 | Putative emu2 | EMU2 | SH2B
- Origin Species
- Human
- SwissProt
- Q96A83
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 45 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MKLALLLPWA CCCLCGSALA TGFLYPFSAA ALQQHGYPEP GAGSPGSGYA SRRHWCHHTV61TRTVSCQVQN GSETVVQRVY QSCRWPGPCA NLVRTLIRPT YRVSYRTVTV LEWRCCPGFT121GSNCDEECMN CTRLSDMSER LTTLEAKVLL LEAAERPSSP DNDLPAPEST PPTWNEDFLP181DAIPLAHPVP RQRRPTGPAG PPGQTGPPGP AGPPGSKGDR GQTGEKGPAG PPGLLGPPGP241RGLPGEMGRP GPPGPPGPAG NPGPSPNSPQ GALYSLQPPT DKDNGDSRLA SAIVDTVLAG301VPGPRGPPGP PGPPGPRGPP GPPGTPGSQG LAGERGTVGP SGEPGVKGEE GEKAATAEGE361GVQQLREALK ILAERVLILE HMIGIHDPLA SPEGGSGQDA ALRANLKMKR GGAQPDGVLA421ALLGPDPGQK SVDQASSRKTRTRPLEQKLISEEDLAANDILDYKDDDDKV
439 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.