Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
COL21A1 / Collagen XXI Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G109373-100
Catalog Number: LS-G109373
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human COL21A1 (NM_030820), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Alpha 1 chain-like collagen | Alpha 1 type XXI collagen | COL1AL | COL21A1 | COLA1L | Collagen alpha-1(XXI) chain | DJ708F5.1 | DJ682J15.1 | FP633 | Collagen | type XXI | alpha 1
- Origin Species
- Human
- NCBI GenBank
- NM_030820
- SwissProt
- Q96P44
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 96.8 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MAHYITFLCM VLVLLLQNSV LAEDGEVRSS CRTAPTDLVF ILDGSYSVGP ENFEIVKKWL61VNITKNFDIG PKFIQVGVVQ YSDYPVLEIP LGSYDSGEHL TAAVESILYL GGNTKTGKAI121QFALDYLFAK SSRFLTKIAV VLTDGKSQDD VKDAAQAARD SKITLFAIGV GSETEDAELR181AIANKPSSTY VFYVEDYIAI SKIREVMKQK LCEESVCPTR IPVAARDERG FDILLGLDVN241KKVKKRIQLS PKKIKGYEVT SKVDLSELTS NVFPEGLPPS YVFVSTQRFK VKKIWDLWRI301LTIDGRPQIA VTLNGVDKIL LFTTTSVING SQVVTFANPQ VKTLFDEGWH QIRLLVTEQD361VTLYIDDQQI ENKPLHPVLG ILINGQTQIG KYSGKEETVQ FDVQKLRIYC DPEQNNRETA421CEIPGFNGEC LNGPSDVGST PAPCICPPGK PGLQGPKGDP GLPGNPGYPG QPGQDGKPGY481QGIAGKPGVP GSPGIQGARG LPGYKGEPGR DGDKGDRGLP GFPGLHGMPG SKGEMGAKGD541KGSPGFYGKK GAKGEKGNAG FPGLPGPAGE PGRHGKDGLM GSPGFKGEAG SPGAPGQDGT601RGEPGIPGFP GNRGLMGQKG EIGPPGQQGK KGAPGMPGLM GSNGSPGQPG TPGSKGSKGE661PGIQGMPGAS GLKGEPGATG SPGEPGYMGL PGIQGKKGDK GNQGEKGIQG QKGENGRQGI721PGQQGIQGHH GAKGERGEKG EPGVRGAIGS KGESGVDGLM GPAGPKGQPG DPGPQGPPGL781DGKPGREFSE QFIRQVCTDV IRAQLPVLLQ SGRIRNCDHC LSQHGSPGIP GPPGPIGPEG841PRGLPGLPGR DGVPGLVGVP GRPGVRGLKG LPGRNGEKGS QGFGYPGEQG PPGPPGPEGP901PGISKEGPPG DPGLPGKDGD HGKPGIQGQP GPPGICDPSL CFSVIARRDP FRKGPNYTRTRPLEQKLISEEDLAANDILDYKDDDDKV
957 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.