Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
COL15A1 / Collagen XV Alpha 1 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G118365-100
Catalog Number: LS-G118365
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Collagen XV (COL15A1) (NM_001855), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- COL15A1 | Collagen alpha-1(XV) chain | Collagen | type XV | alpha 1 | Collagen alpha 1(xv) | Collagen type XV proteoglycan
- Origin Species
- Human
- NCBI GenBank
- NM_001855
- SwissProt
- P39059
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 141.72 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MAPRRNNGQC WCLLMLLSVS TPLPAVTQTR GATETASQGH LDLTQLIGVP LPSSVSFVTG61YGGFPAYSFG PGANVGRPAR TLIPSTFFRD FAISVVVKPS STRGGVLFAI TDAFQKVIYL121GLRLSGVEDG HQRIILYYTE PGSHVSQEAA AFSVPVMTHR WNRFAMIVQG EEVTLLVNCE181EHSRIPFQRS SQALAFESSA GIFMGNAGAT GLERFTGSLQ QLTVHPDPRT PEELCDPEES241SASGETSGLQ EADGVAEILE AVTYTQASPK EAKVEPINTP PTPSSPFEDM ELSGEPVPEG301TLETTNMSII QHSSPKQGSG EILNDTLEGV HSVDGDPITD SGSGAGAFLD IAEEKNLAAT361AAGLAEVPIS TAGEAEASSV PTGGPTLSMS TENPEEGVTP GPDNEERLAA TAAGEAEALA421SMPGEVEASG VAPGELDLSM SAQSLGEEAT VGPSSEDSLT TAAAATEVSL STFEDEEASG481VPTDGLAPLT ATMAPERAVT SGPGDEEDLA AATTEEPLIT AGGEESGSPP PDGPPLPLPT541VAPERWITPA QREHVGMKGQ AGPKGEKGDA GEELPGPPEP SGPVGPTAGA EAEGSGLGWG601SDVGSGSGDL VGSEQLLRGP PGPPGPPGLP GIPGKPGTDV FMGPPGSPGE DGPAGEPGPP661GPEGQPGVDG ATGLPGMKGE KGARGPNGSV GEKGDPGNRG LPGPPGKKGQ AGPPGVMGPP721GPPGPPGPPG PGCTMGLGFE DTEGSGSTQL LNEPKLSRPT AAIGLKGEKG DRGPKGERGM781DGASIVGPPG PRGPPGHIKV LSNSLINITH GFMNFSDIPE LVGPPGPDGL PGLPGFPGPR841GPKGDTGLPG FPGLKGEQGE KGEPGAILTE DIPLERLMGK KGEPGMHGAP GPMGPKGPPG901HKGEFGLPGR PGRPGLNGLK GTKGDPGVIM QGPPGLPGPP GPPGPPGAVI NIKGAIFPIP961VRPHCKMPVD TAHPGSPELI TFHGVKGEKG SWGLPGSKGE KGDQGAQGPP GPPLDLAYLR1021HFLNNLKGEN GDKGFKGEKG EKGDINGSFL MSGPPGLPGN PGPAGQKGET VVGPQGPPGA1081PGLPGPPGFG RPGDPGPPGP PGPPGPPAIL GAAVALPGPP GPPGQPGLPG SRNLVTAFSN1141MDDMLQKAHL VIEGTFIYLR DSTEFFIRVR DGWKKLQLGE LIPIPADSPP PPALSSNPHQ1201LLPPPNPISS ANYEKPALHL AALNMPFSGD IRADFQCFKQ ARAAGLLSTY RAFLSSHLQD1261LSTIVRKAER YSLPIVNLKG QVLFNNWDSI FSGHGGQFNM HIPIYSFDGR DIMTDPSWPQ1321KVIWHGSSPH GVRLVDNYCE AWRTADTAVT GLASPLSTGK ILDQKAYSCA NRLIVLCIEN1381SFMTDARKTRTRPLEQKLISEEDLAANDILDYKDDDDKV
1388 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.