Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
COL14A1 / Collagen XIV Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G110769-100
Catalog Number: LS-G110769
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human COL14A1 (NM_021110), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Collagen | type XIV | alpha 1 | Undulin | COL14A1 | Collagen alpha-1(XIV) chain
- Origin Species
- Human
- NCBI GenBank
- NM_021110
- SwissProt
- Q05707
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 193.5 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MKIFQRKMRY WLLPPFLAIV YFCTIVQGQV APPTRLRYNV ISHDSIQISW KAPRGKFGGY61KLLVTPTSGG KTNQLNLQNT ATKAIIQGLM PDQNYTVQII AYNKDKESKP AQGQFRIKDL121EKRKDPKPRV KVVDRGNGSR PSSPEEVKFV CQTPAIADIV ILVDGSWSIG RFNFRLVRHF181LENLVTAFDV GSEKTRIGLA QYSGDPRIEW HLNAFSTKDE VIEAVRNLPY KGGNTLTGLA241LNYIFENSFK PEAGSRTGVS KIGILITDGK SQDDIIPPSR NLRESGVELF AIGVKNADVN301ELQEIASEPD STHVYNVAEF DLMHTVVESL TRTLCSRVEE QDREIKASAH AITGPPTELI361TSEVTARSFM VNWTHAPGNV EKYRVVYYPT RGGKPDEVVV DGTVSSTVLK NLMSLTEYQI421AVFAIYAHTA SEGLRGTETT LALPMASDLL LYDVTENSMR VKWDAVPGAS GYLILYAPLT481EGLAGDEKEM KIGETHTDIE LSGLLPNTEY TVTVYAMFGE EASDPVTGQE TTLALSPPRN541LRISNVGSNS ARLTWDPTSR QINGYRIVYN NADGTEINEV EVDPITTFPL KGLTPLTEYT601IAIFSIYDEG QSEPLTGVFT TEEVPAQQYL EIDEVTTDSF RVTWHPLSAD EGLHKLMWIP661VYGGKTEEVV LKEEQDSHVI EGLEPGTEYE VSLLAVLDDG SESEVVTAVG TTLDSFWTEP721ATTIVPTTSV TSVFQTGIRN LVVGDETTSS LRVKWDISDS DVQQFRVTYM TAQGDPEEEV781IGTVMVPGSQ NNLLLKPLLP DTEYKVTVTP IYTDGEGVSV SAPGKTLPSS GPQNLRVSEE841WYNRLRITWD PPSSPVKGYR IVYKPVSVPG PTLETFVGAD INTILITNLL SGMDYNVKIF901ASQASGFSDA LTGMVKTLFL GVTNLQAKHV EMTSLCAHWQ VHRHATAYRV VIESLQDRQK961QESTVGGGTT RHCFYGLQPD SEYKISVYTK LQEIEGPSVS IMEKTQSLPT RPPTFPPTIP1021PAKEVCKAAK ADLVFMVDGS WSIGDENFNK IISFLYSTVG ALNKIGTDGT QVAMVQFTDD1081PRTEFKLNAY KTKETLLDAI KHISYKGGNT KTGKAIKYVR DTLFTAESGT RRGIPKVIVV1141ITDGRSQDDV NKISREMQLD GYSIFAIGVA DADYSELVSI GSKPSARHVF FVDDFDAFKK1201IEDELITFVC ETASATCPVV HKDGIDLAGF KMMEMFGLVE KDFSSVEGVS MEPGTFNVFP1261CYQLHKDALV SQPTRYLHPE GLPSDYTISF LFRILPDTPQ EPFALWEILN KNSDPLVGVI1321LDNGGKTLTY FNYDQSGDFQ TVTFEGPEIR KIFYGSFHKL HIVVSETLVK VVIDCKQVGE1381KAMNASANIT SDGVEVLGKM VRSRGPGGNS APFQLQMFDI VCSTSWANTD KCCELPGLRD1441DESCPDLPHS CSCSETNEVA LGPAGPPGGP GLRGPKGQQG EPGPKGPDGP RGEIGLPGPQ1501GPPGPQGPSG LSIQGMPGMP GEKGEKGDTG LPGPQGIPGG VGSPGRDGSP GQRGLPGKDG1561SSGPPGPPGP IGIPGTPGVP GITGSMGPQG ALGPPGVPGA KGERGERGDL QSQAMVRSVA1621RQVCEQLIQS HMARYTAILN QIPSHSSSIR TVQGPPGEPG RPGSPGAPGE QGPPGTPGFP1681GNAGVPGTPG ERGLTGIKGE KGNPGVGTQG PRGPPGPAGP SGESRPGSPG PPGSPGPRGP1741PGHLGVPGPQ GPSGQPGYCD PSSCSAYGVR APHPDQPEFT PVQDELEAME LWGPGVTRTRPLEQKLISEEDLAANDILDYKDDDDKV
1796 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.